Reliable Diagnostic Supplies (Pty) Ltd (Johannesburg, South Africa) has been appointed distributor for Dr. Fooke Laboratorien GmbH range of in-vitro Allergy and Autoimmune Diagnostics in South Africa.
The worldwide frequency of allergies has increased significantly over the past decades. Allergic Rhinitis on its own affects 10-30% of the population! The term allergy is often used for type I hypersensitivity reactions (immediate type reactions), whose symptoms generally occur within 30-60 minutes after contact with the allergen. The most frequent symptoms are: hay fever (rhinitis), conjunctivitis, hives (urticaria), allergic asthma and as the most dangerous manifestation anaphylaxis (the anaphylactic shock).
ALFA (Allergy Lateral Flow Assay) is a rapid test for the qualitative determination of allergen specific Immunoglobulin E (sIgE) in human whole blood, serum, or plasma.
ALFA consists of a uniform test device - the ALFA Basis Set - in combination with several arbitrary single- or allergen-mixture-solutions (also called "screening mixtures"). Two different test systems are available, a single-strip or an eight-strip cassette.
The sample (serum, plasma or whole blood) is transferred onto the sample application point of the ALFA Basis Set. Immediately afterwards the desired allergen solution is added. After 20 minutes the result can be evaluated based on
the test line (T). The functionality of the test is evaluated based on the control line (C).
By choosing the right allergen solution, patient and symptom-specific testing in a point-of-care setting is feasible.
Amongst others, the following single-allergens and allergen mixtures are available:
House dust mites (Dermatophagoides pteronyssinus, d1; Dermatophagoides farinae, d2), Birch (t3), Timothy Grass (g6), Cat (e1), Dog (e5), Alternaria alternata (m6).
Seasonal Screen: Timothy Grass (g6), Alternaria alternata (m6), Birch (t3), Mugwort (w6).
Perennial Screen: Mites (D. pteronyssinus, d1; D. farinae, d2), German Cockroach (i6), Aspergillus fumigatus (m3).
Further allergens and allergen screens can be found in the current list of ALFA allergens.
• Short assay time (result within just 20 min)
• Serum, plasma or whole blood sample can be used for testing - can be used in Doctor's rooms or pharmacy clinics!
• Excellent correlation with skin prick test and other in-vitro sIgE test methods - Please speak to us if interested in some publications and literature. We listed a few below.
PERFORMANCE AGAINST SKIN PRICK TEST
The concordance between ALFA and skin prick test is shown in figure 2 below:
PERFORMANCE AGAINST OTHER ALLERGY IVD TESTING SYSTEMS
Sensitivity and specificity of the ALFA was determined against different in-vitro diagnostic test methods (ImmunoCAP® and ALLERG-OLIQ). Sensitivity was calculated based on a cutoff value of 0.7 IU/mL. Please check figure 3 below:
1. Hamilton RG, Franklin Adkinson N Jr: In-vitro assays for the diagnosis of IgE-mediated disorders. J Allergy Clin Immunol 2004; 114: 213-25.
2. Lucassen R, Fooke M, Kleine-Tebbe J, Mahler M: Development and Evaluation of a Rapid Assay for the Diagnosis of IgE-mediated Type I Allergies. J Investig Allergol Clin Immunol. 2008; 18 (3):223-30.
3. Lucassen R, Fooke M, Lorenz C, Kleine-Tebbe J, Mahler M: Evaluation of a rapid assay for the diagnosis of type I allergy. Abstract: EAACI 2008 Barcelona, Spain.
DOWNLOAD PRODUCT INFORMATION
ALFA Rapid Test Pack Insert: Instructions of Use leaflet with detailed information on how to perform the ALFA Allergy Test
ALLERG-O-LIQ Reversed Enzyme Allergo Sorbent Test
ALLERG-O-LIQ i1/i3 Performance specific IgE against bee- (i1) and wasp venom (i3)
CCD Determination of CCD-Antibodies
Molecular Allergy Diagnostics Recombinant and native allergens in Type I Allergy Diagnostics
Full Catalogue of Dr. Fooke Laboratorien Allergy and Autoimmune Diagnostics